4I6X
Crystal Structure of Non-catalyic Domain of Protein Disulfide Isomerase-related (PDIr) Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-21 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9770 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.686, 41.265, 90.893 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.450 - 1.500 |
R-factor | 0.1933 |
Rwork | 0.191 |
R-free | 0.23681 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.170 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 19332 | |
<I/σ(I)> | 43.5 | 3.6 |
Completeness [%] | 95.1 | 67.01 |
Redundancy | 5.7 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 0.15 M DL-malic acid, 20% PEG3350, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 294K |