4I6V
The crystal structure of an amidohydrolase 2 from Planctomyces limnophilus DSM 3776
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97915 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.224, 111.245, 147.148 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.893 - 2.137 |
R-factor | 0.1705 |
Rwork | 0.168 |
R-free | 0.21810 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.072 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.000 | 2.190 |
High resolution limit [Å] | 2.137 | 2.150 |
Rmerge | 0.112 | 0.665 |
Number of reflections | 73652 | |
<I/σ(I)> | 25.4 | 2.6 |
Completeness [%] | 98.8 | 90.2 |
Redundancy | 6 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 297 | 0.1 HEPES:NaOH, 25% (w/v) PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |