4I69
Crystal structure of the K463A mutant of the RRM domain of RNA helicase HERA from T. thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-07-25 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0097 |
Spacegroup name | P 31 |
Unit cell lengths | 49.221, 49.221, 78.830 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.627 - 1.790 |
R-factor | 0.2004 |
Rwork | 0.195 |
R-free | 0.24480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3i31 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.310 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_1223)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 78.800 | 1.900 |
High resolution limit [Å] | 1.790 | 1.790 |
Rmerge | 0.092 | 0.587 |
Number of reflections | 19275 | |
<I/σ(I)> | 8.9 | 1.1 |
Completeness [%] | 95.6 | |
Redundancy | 3.3 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |