4I64
3-hydroxy-3-methylglutaryl Coenzyme A reductase from Pseudomonas mevalonii, a high resolution native structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-06-28 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9 |
Spacegroup name | I 41 3 2 |
Unit cell lengths | 225.613, 225.613, 225.613 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.620 - 1.750 |
R-factor | 0.19914 |
Rwork | 0.198 |
R-free | 0.24630 |
Structure solution method | MIR |
RMSD bond length | 0.005 |
RMSD bond angle | 0.928 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.720 | 1.860 |
High resolution limit [Å] | 1.750 | 1.750 |
Number of reflections | 94406 | |
Completeness [%] | 98.2 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.2M ammonium sulfate, 75mM sodium citrate, 100 mM sodium ADA microseeding, pH 293, VAPOR DIFFUSION, SITTING DROP, temperature 6.5K |