4I5V
Crystal structure of yeast Ap4A phosphorylase Apa2 in complex with Ap4A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97923 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 203.353, 52.781, 70.764 |
Unit cell angles | 90.00, 99.92, 90.00 |
Refinement procedure
Resolution | 42.070 - 2.696 |
R-factor | 0.185 |
Rwork | 0.181 |
R-free | 0.26080 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.330 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.790 |
High resolution limit [Å] | 2.696 | 2.700 |
Rmerge | 0.070 | 0.348 |
Number of reflections | 19447 | |
<I/σ(I)> | 7.4 | 1.9 |
Completeness [%] | 93.9 | 96.7 |
Redundancy | 2.2 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 23% PEG 2K, 0.1M Tris-HCl, pH 8.0, 20% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 289K |