4I5R
Crystal structure of a fungal chimeric cellobiohydrolase Cel6A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.033 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 158.610, 45.262, 58.602 |
| Unit cell angles | 90.00, 95.21, 90.00 |
Refinement procedure
| Resolution | 35.309 - 1.500 |
| R-factor | 0.1557 |
| Rwork | 0.154 |
| R-free | 0.18080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.515 |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.309 | 35.309 | 1.580 |
| High resolution limit [Å] | 1.500 | 4.740 | 1.500 |
| Rmerge | 0.027 | 0.365 | |
| Total number of observations | 7018 | 30476 | |
| Number of reflections | 65106 | ||
| <I/σ(I)> | 16.1 | 21.7 | 2.1 |
| Completeness [%] | 97.9 | 97.1 | 97.7 |
| Redundancy | 3.4 | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.75 | pH 5.75, VAPOR DIFFUSION, SITTING DROP |
