4I5O

Crystal Structure of W-W-R ClpX Hexamer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	55.199, 199.878, 222.279
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.319 - 4.479
R-factor	0.2879
Rwork	0.286
R-free	0.32330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hws
RMSD bond length	0.002
RMSD bond angle	0.452
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.5_2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	4.660
High resolution limit [Å]	4.479	9.670	4.479
Rmerge	0.077	0.041	0.278
Number of reflections	14285
<I/σ(I)>	9.6
Completeness [%]	92.2	95.6	80.4
Redundancy	4	4.2	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	9	298	2.2 M ammonium sulfate, 0.2 M ammonium bromide, 0.1 M Bicine, pH 9.0, VAPOR DIFFUSION, temperature 298K