4I45
Crystal Structure of Orf6 protein from Photobacterium profundum, Mg2+-bound form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 51.292, 54.362, 88.363 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.300 - 1.400 |
R-factor | 0.15052 |
Rwork | 0.148 |
R-free | 0.19606 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3r87 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.608 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0111) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.300 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Number of reflections | 23821 | |
Completeness [%] | 96.3 | 76.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.05 M magnesium chloride hexahydrate, 0.1 M HEPES pH 7.5, 30% v/v Polyethylene glycol monomethyl ether 550, VAPOR DIFFUSION, HANGING DROP, temperature 298K |