4I37
Crystal structure of rabbit ryanodine receptor 1 (residues 1-536) disease mutant R402G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Detector technology | CCD |
Collection date | 2010-09-08 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97949 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 118.258, 118.258, 243.934 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.820 - 2.950 |
R-factor | 0.25251 |
Rwork | 0.251 |
R-free | 0.28950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xoa |
RMSD bond length | 0.007 |
RMSD bond angle | 1.004 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.790 | 4.660 | 3.110 |
High resolution limit [Å] | 2.950 | 4.170 | 2.950 |
Number of reflections | 22009 | ||
Completeness [%] | 99.8 | 100 | 100 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6.5 | pH 6.5, EVAPORATION |