4I34
Crystal Structure of W-W-W ClpX Hexamer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.341, 199.606, 203.441 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.337 - 4.122 |
R-factor | 0.2761 |
Rwork | 0.274 |
R-free | 0.30770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hws |
RMSD bond length | 0.003 |
RMSD bond angle | 0.499 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.5_2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 4.300 |
High resolution limit [Å] | 4.122 | 8.930 | 4.122 |
Rmerge | 0.059 | 0.034 | 0.294 |
Number of reflections | 18530 | ||
<I/σ(I)> | 11.1 | ||
Completeness [%] | 97.2 | 98.2 | 84.1 |
Redundancy | 5.7 | 5.6 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.8 | 298 | 75 mM sodium acetate, 1.9 M ammonium sulfate, pH 4.8, VAPOR DIFFUSION, temperature 298K |