4I2W
Crystal structure of the myosin chaperone UNC-45 from C.elegans in complex with a Hsp70 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-08 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 86.276, 86.276, 808.978 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.817 - 3.600 |
| Rwork | 0.234 |
| R-free | 0.28450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data scaling software | SCALA (3.3.16) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 47.817 | 3.790 |
| High resolution limit [Å] | 3.600 | 11.380 | 3.600 |
| Rmerge | 0.053 | 0.697 | |
| Total number of observations | 4280 | 18942 | |
| Number of reflections | 22485 | ||
| <I/σ(I)> | 8.5 | 11.6 | 1.1 |
| Completeness [%] | 99.9 | 98.3 | 100 |
| Redundancy | 6.1 | 4.7 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | 0.1 M HEPES, 10% PEG 8000, 12% ethylene glycol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
