4I2S

Crystal structure of rabbit ryanodine receptor 1 (residues 1-536) disease mutant I404M

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Detector technology	CCD
Collection date	2010-09-08
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97949
Spacegroup name	H 3 2
Unit cell lengths	171.374, 171.374, 299.429
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.890 - 2.500
R-factor	0.23296
Rwork	0.232
R-free	0.24911
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2x0a
RMSD bond length	0.006
RMSD bond angle	0.942
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.600	4.560	2.640
High resolution limit [Å]	2.500	3.950	2.500
Rmerge	0.097
Number of reflections	58584
Completeness [%]	100.0	100	100
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8		pH 8.0, EVAPORATION