4I2N
Crystal structure of 31kD Heat Shock Protein, VcHsp31 from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-06-20 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.514, 79.094, 133.132 |
| Unit cell angles | 90.00, 95.22, 90.00 |
Refinement procedure
| Resolution | 29.540 - 1.900 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | AUTOMAR |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.990 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.280 | |
| Number of reflections | 113580 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 98.1 | 91 |
| Redundancy | 1.79 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 293 | 5% PEG6000, 8% MPD, pH 5.0, VAPOR DIFFUSION, temperature 293K |
