4I2C
Ternary complex of mouse TdT with ssDNA and AMPcPP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9725 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.875, 84.763, 116.482 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.450 - 2.100 |
R-factor | 0.1832 |
Rwork | 0.181 |
R-free | 0.21720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jms |
RMSD bond length | 0.010 |
RMSD bond angle | 1.000 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.107 | 0.706 |
Number of reflections | 27460 | |
<I/σ(I)> | 2.2 | |
Completeness [%] | 99.0 | 100 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20-26% PEG4000, 100 mM HEPES, 200 mM ammonium formate, pH 6.0-7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |