4I1Q

Crystal Structure of hBRAP1 N-BAR domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-07
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	79.147, 81.278, 81.101
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.410 - 2.530
R-factor	0.19891
Rwork	0.196
R-free	0.25220
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.741
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC (5.7.0024)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.410	2.730
High resolution limit [Å]	2.530	2.530
Rmerge	0.083	0.386
Number of reflections	15899
<I/σ(I)>	13.9	2.9
Completeness [%]	94.9	96.5
Redundancy	4.6	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.3	293	10% MPD, 7% PEG 4000, 0.1M sodium citrate, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
1	VAPOR DIFFUSION, HANGING DROP	5.3	293	10% MPD, 7% PEG 4000, 0.1M sodium citrate, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
1	VAPOR DIFFUSION, HANGING DROP	5.3	293	10% MPD, 7% PEG 4000, 0.1M sodium citrate, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K