4I1P
Human MALT1 (caspase-IG3) in complex with activity based-probe
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-03-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 178.243, 53.482, 78.740 |
Unit cell angles | 90.00, 103.80, 90.00 |
Refinement procedure
Resolution | 43.939 - 2.403 |
R-factor | 0.2007 |
Rwork | 0.198 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.307 |
Data reduction software | DENZO |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.000 | 2.550 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 27391 | |
Completeness [%] | 91.1 | 69.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 25 mM Mes, 75 mM calcium acetate, 10 % benzamidine hypochloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |