4I1M
Crystal structure of the Legionella pneumophila GAP domain of LepB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-07-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 152.700, 152.700, 129.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.831 - 2.804 |
R-factor | 0.2015 |
Rwork | 0.200 |
R-free | 0.23800 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.676 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SOLVE (2.13) |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.831 | 2.870 | |
High resolution limit [Å] | 2.800 | 12.520 | 2.800 |
Rmerge | 0.081 | 0.031 | 0.737 |
Number of reflections | 38042 | 499 | 2773 |
<I/σ(I)> | 24.36 | 66.89 | 3.51 |
Completeness [%] | 100.0 | 97.8 | 100 |
Redundancy | 13.29 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M Bicine, pH 7.5, 0.75 M lithium chloride, 6% PEG6000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M Bicine, pH 7.5, 0.75 M lithium chloride, 6% PEG6000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |