4I1K

Crystal Structure of VRN1 (Residues 208-341)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-16
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.956
Spacegroup name	C 1 2 1
Unit cell lengths	106.152, 47.310, 61.413
Unit cell angles	90.00, 114.97, 90.00

Refinement procedure

Resolution	50.000 - 1.600
Rwork	0.120
R-free	0.16600
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX ((phenix.refine: dev_1031))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.600
Number of reflections	34680
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	19 mg/mL protein in 10 mM HEPES, pH 7.0, 50 mM sodium chloride, 5 mM DTT + equal volume well solution (0.1 M MES, pH 6.0, 1.4 M sodium chloride, 10 mM manganese chloride), VAPOR DIFFUSION, HANGING DROP, temperature 277K