4I1G

dna octamer d(GTseGTACAC) partially crosslinked with two platinums

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	200
Detector technology	CCD
Collection date	2012-09-27
Detector	CUSTOM-MADE
Wavelength(s)	0.97
Spacegroup name	P 43 21 2
Unit cell lengths	42.368, 42.368, 24.056
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.900 - 1.250
R-factor	0.20947
Rwork	0.209
R-free	0.21729
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dns
RMSD bond length	0.040
RMSD bond angle	3.373
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall
Low resolution limit [Å]	15.900
High resolution limit [Å]	1.250
Rmerge	0.094
Number of reflections	5900
<I/σ(I)>	25.6
Completeness [%]	94.3
Redundancy	11.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	10% MPD, 40 mM Sodium codylate trihydrate, 12 mM Spermine tetrahydrochloride, 80 mM Strontium chloride , pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K