Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2010-11-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.10 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.260, 147.100, 95.959 |
| Unit cell angles | 90.00, 106.49, 90.00 |
Refinement procedure
| Resolution | 43.909 - 3.200 |
| R-factor | 0.1906 |
| Rwork | 0.188 |
| R-free | 0.24190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 2BC4 (DM) 1KLU (DR1) |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.853 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.314 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.683 | 0.683 |
| Number of reflections | 36565 | |
| <I/σ(I)> | 5.4 | 5.4 |
| Completeness [%] | 93.9 | 94 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 100 mM Na acetate, 8% PEG 4K, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
