4I0O
Nucleoporin ELYS (aa1-494), Mus musculus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.598, 78.117, 58.849 |
| Unit cell angles | 90.00, 106.59, 90.00 |
Refinement procedure
| Resolution | 26.525 - 1.900 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.21450 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.429 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.8_1063) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 5.160 | 1.900 |
| Rmerge | 0.084 | 0.046 | 0.714 |
| Number of reflections | 69665 | ||
| <I/σ(I)> | 10.3 | ||
| Completeness [%] | 98.9 | 97.7 | 97 |
| Redundancy | 2.9 | 2.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | under oil | 6.5 | 292 | Bis-Tris/HCl, PEG 3350, ammonium acetate, Octyl-beta-D-glucopyranoside, pH 6.5, under oil, temperature 292K |
