4I0B
structure of the mutant Catabolite gene activator protein H160L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9687 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.780, 72.320, 58.410 |
| Unit cell angles | 90.00, 109.89, 90.00 |
Refinement procedure
| Resolution | 54.900 - 1.500 |
| R-factor | 0.18172 |
| Rwork | 0.180 |
| R-free | 0.20833 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.601 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.900 | 1.539 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 67230 | |
| Completeness [%] | 99.7 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 7-10% Peg3350, 15-20% MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
