4HZY

Crystal structure of influenza A neuraminidase N3-H274Y

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-01-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	I 4
Unit cell lengths	106.413, 106.413, 62.251
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.651 - 1.598
R-factor	0.1523
Rwork	0.151
R-free	0.17120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bat
RMSD bond length	0.007
RMSD bond angle	1.187
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.598	1.600
Number of reflections	44906
Completeness [%]	99.5	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	291	20mM Tris, pH 8.0, 50mM NaCl, 0.1M BIS-TRIS propane (pH 9.0), 10% v/v Jeffamine ED-2001 (pH 7.0) , VAPOR DIFFUSION, temperature 291K