4HZS
Crystal structure of Ack1 kinase domain with C-terminal SH3 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 98 |
| Detector technology | PIXEL |
| Collection date | 2010-03-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 4 |
| Unit cell lengths | 145.463, 145.463, 103.745 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.040 - 3.230 |
| R-factor | 0.247 |
| Rwork | 0.246 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.100 |
| Data scaling software | autoPROC |
| Phasing software | CNX |
| Refinement software | CNX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.040 | 3.400 |
| High resolution limit [Å] | 3.230 | 3.230 |
| Rmerge | 0.187 | 0.477 |
| Number of reflections | 34878 | |
| <I/σ(I)> | 9.9 | 2 |
| Completeness [%] | 99.5 | 96.7 |
| Redundancy | 6.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 286 | 0.2 M ammonium sulfate, 0.1 M Bis-Tris, pH 6.6, 22-24% PEG3350, 10-20 mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 286K |
