4HZP
The Structure of the Bifunctional Acetyltransferase/Decarboxylase LnmK from the Leinamycin Biosynthetic Pathway Revealing Novel Activity for a Double Hot Dog Fold
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-12-10 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.97857 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 59.711, 59.711, 311.113 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.720 - 1.770 |
R-factor | 0.2274 |
Rwork | 0.225 |
R-free | 0.27477 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hzn |
RMSD bond length | 0.022 |
RMSD bond angle | 1.980 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 1.770 |
Rmerge | 0.107 |
Number of reflections | 32885 |
<I/σ(I)> | 14.7 |
Completeness [%] | 98.5 |
Redundancy | 9.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | Protein: 20 mg/mL in 100 mM NaCl and 10 mM Tris-HCl, pH 8.0 plus 10 mM methylmalonyl-CoA. Precipitant: 20 - 23% MEPEG 2000, 0.25-0.35 M (NH4)2SO4 and 0.1 M Bis-tris propane-HCl, pH 7.0 in 4 L drops (1:1, protein:well). , VAPOR DIFFUSION, HANGING DROP, temperature 298K |