4HZF
structure of the wild type Catabolite gene Activator Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-02-27 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.500, 101.850, 53.770 |
Unit cell angles | 90.00, 111.06, 90.00 |
Refinement procedure
Resolution | 50.900 - 1.480 |
R-factor | 0.19727 |
Rwork | 0.195 |
R-free | 0.24152 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.636 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.900 | 1.518 |
High resolution limit [Å] | 1.480 | 1.480 |
Number of reflections | 71204 | |
Completeness [%] | 98.8 | 99.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 10% (w/v) polyethylene glycol 3350 and 15-20% (v/v) 2-methyl-2,4-pentanediol , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |