4HYZ

Crystal structure of a DUF3887 family protein (RUMGNA_01855) from Ruminococcus gnavus ATCC 29149 at 2.25 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-06-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97871,0.97922,0.91837
Spacegroup name	P 65 2 2
Unit cell lengths	66.290, 66.290, 253.830
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.377 - 2.250
R-factor	0.1876
Rwork	0.186
R-free	0.20840
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.060
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.377	29.377	2.310
High resolution limit [Å]	2.250	10.060	2.250
Rmerge		0.040	0.873
Total number of observations		2190	11870
Number of reflections	16696
<I/σ(I)>	15	16	0.9
Completeness [%]	99.9	96.3	100
Redundancy	11.1	8.6	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	277	2.40M ammonium sulfate, 0.1M citric acid pH 4.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K