4HYN
X-ray crystal structure of Thermotoga maritima FliY
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-05 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97670 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.750, 85.890, 119.940 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.500 |
R-factor | 0.21 |
Rwork | 0.210 |
R-free | 0.26000 |
Structure solution method | SAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.500 |
Number of reflections | 33692 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.2 M Ammonium sulfate, 30% PEG 8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |