4HYG
Structure of a presenilin family intramembrane aspartate protease in C222 space group
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-24 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.07171, 1.05384, 1.07206 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 168.302, 201.892, 117.534 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.108 - 3.320 |
R-factor | 0.3007 |
Rwork | 0.298 |
R-free | 0.34410 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.625 |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.300 |
Number of reflections | 24686 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.6 | 291 | 0.1M Glycine, 0.2M (NH4)2SO4, 20%(w/v) PEG500MME, 6%(w/v) Glycerol, 0.04%(w/v) Anapoe-C12E8, pH 3.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |