4HYE

Crystal structure of a response regulator spr1814 from Streptococcus pneumoniae reveals unique interdomain contacts among NarL family proteins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	DIFFRACTOMETER
Collection date	2009-03-17
Detector	ADSC QUANTUM 310
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	46.910, 71.376, 117.727
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.450 - 1.700
R-factor	0.2
Rwork	0.198
R-free	0.24150
Structure solution method	SAD
RMSD bond length	0.020
RMSD bond angle	1.897
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.760
High resolution limit [Å]	1.700	3.660	1.700
Rmerge	0.065	0.038	0.292
Number of reflections	42035
<I/σ(I)>	11.4
Completeness [%]	94.7	97.9	67
Redundancy	8.1	12.6	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	295	0.1M sodium citrate tribasic dehydrate pH 5.0, 18% (v/v) Jeffamine ED-2001 pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K