4HYC
Structure of a presenilin family intramembrane aspartate protease in P2 space group
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.07171, 1.05384, 1.07206 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 127.224, 115.827, 137.173 |
| Unit cell angles | 90.00, 100.59, 90.00 |
Refinement procedure
| Resolution | 40.161 - 3.950 |
| R-factor | 0.3108 |
| Rwork | 0.308 |
| R-free | 0.34100 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.494 |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.950 |
| Number of reflections | 27265 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.6 | 291 | 0.1 M Glycine pH 3.6, 0.2 M (NH4)2SO4, 20% (w/v) PEG500MME, 6% (w/v) Glycerol and 0.04% (w/v) Anapoe-C12E8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
