4HYB
Crystal structure of the substrate binding domain of E.coli DnaK in complex with pyrrhocoricin_LYZI (residues 1 to 10)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-29 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.918 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 77.450, 162.168, 45.208 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.600 - 1.700 |
R-factor | 0.21299 |
Rwork | 0.211 |
R-free | 0.24410 |
Structure solution method | PDB ENTRY 3DPO |
RMSD bond length | 0.021 |
RMSD bond angle | 2.065 |
Data reduction software | XDS |
Data scaling software | SCALA |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.600 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 60893 | |
Completeness [%] | 95.6 | 99.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 292 | 2.4 M ammonium sulfate, 0.1 M sodium acetate pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |