4HXT
Crystal Structure of Engineered Protein. Northeast Structural Genomics Consortium Target OR329
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.493, 31.941, 72.543 |
| Unit cell angles | 90.00, 90.78, 90.00 |
Refinement procedure
| Resolution | 40.130 - 1.950 |
| R-factor | 0.166 |
| Rwork | 0.164 |
| R-free | 0.19000 |
| Structure solution method | MIR |
| Starting model (for MR) | 4db8 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.918 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.040 |
| High resolution limit [Å] | 1.950 | 1.970 |
| Rmerge | 0.078 | 0.254 |
| Number of reflections | 28434 | |
| <I/σ(I)> | 25.7 | 3.4 |
| Completeness [%] | 91.5 | 58.3 |
| Redundancy | 3.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch crystallization under oil | 4.2 | 277 | 20% PEG 8000, 0.1M magnesium nitrate, 0.1M sodium citrate, pH 4.2, Microbatch crystallization under oil, temperature 277K |
