4HXJ
Crystal structure of SH3:RGT complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97885 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 27.980, 40.830, 43.340 |
Unit cell angles | 90.00, 105.97, 90.00 |
Refinement procedure
Resolution | 29.163 - 2.000 |
R-factor | 0.1445 |
Rwork | 0.139 |
R-free | 0.19250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fmk |
RMSD bond length | 0.007 |
RMSD bond angle | 1.025 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.200 | 2.080 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.170 | 0.360 |
Number of reflections | 6468 | |
<I/σ(I)> | 10 | 7 |
Completeness [%] | 99.0 | 100 |
Redundancy | 8 | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 100mM Tris, pH 8.0, 1.3M sodium citrate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |