4HXC
Crystal structure of a putative glycosyl hydrolase (BACUNI_00951) from Bacteroides uniformis ATCC 8492 at 2.15 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-06-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.91837,0.9792,0.97868 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 91.537, 91.537, 99.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.461 - 2.150 |
R-factor | 0.1857 |
Rwork | 0.184 |
R-free | 0.22210 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.080 |
Data reduction software | XDS |
Data scaling software | XSCALE (March 15, 2012) |
Phasing software | SHELX |
Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.461 | 2.230 | |
High resolution limit [Å] | 2.150 | 4.610 | 2.150 |
Rmerge | 0.073 | 0.027 | 0.421 |
Number of reflections | 22690 | 4236 | 4010 |
<I/σ(I)> | 9.33 | 23.6 | 2.1 |
Completeness [%] | 93.0 | 94.4 | 87.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2M potassium fluoride 20% polyethylene glycol 3350, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |