4HX4
Structure of MNTR mutant E11K complexed with Mn2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-08-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.1159 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.427, 46.081, 74.892 |
Unit cell angles | 90.00, 92.96, 90.00 |
Refinement procedure
Resolution | 30.330 - 1.650 |
R-factor | 0.23534 |
Rwork | 0.234 |
R-free | 0.26444 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2f5d |
RMSD bond length | 0.008 |
RMSD bond angle | 1.120 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.790 | 1.740 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.052 | 0.360 |
Number of reflections | 40214 | |
<I/σ(I)> | 22.2 | 2.2 |
Completeness [%] | 98.8 | 97.4 |
Redundancy | 3.56 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 22% ETHYLENE GLYCOL, 55 MM LITHIUM SULFATE, 2 MM MANGANESE CHLORIDE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |