4HWV

Structure of Pectate Lyase from Acidovorax avenae subsp citrulli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.007
Spacegroup name	P 1 21 1
Unit cell lengths	69.676, 71.872, 90.953
Unit cell angles	90.00, 92.71, 90.00

Refinement procedure

Resolution	44.446 - 1.370
R-factor	0.1257
Rwork	0.124
R-free	0.14950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.275
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall
Low resolution limit [Å]	45.000
High resolution limit [Å]	1.370
Number of reflections	188015

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.3	298	pH 4.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K