4HWL
Crystal Structure Analysis of the Bacteriorhodopsin in Facial Amphiphile-7 DMPC Bicelle
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97945 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.160, 113.290, 56.190 |
Unit cell angles | 90.00, 114.27, 90.00 |
Refinement procedure
Resolution | 42.080 - 2.000 |
R-factor | 0.15942 |
Rwork | 0.159 |
R-free | 0.17183 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kme |
RMSD bond length | 0.021 |
RMSD bond angle | 1.879 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASES |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.670 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.074 | 0.550 |
Number of reflections | 34192 | |
<I/σ(I)> | 6.5 | 1.7 |
Completeness [%] | 96.6 | 95.1 |
Redundancy | 2.1 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 3.4 | 300.15 | 3.6M Sodium Phosphate (pH 3.4), 3.5% Triethyleneglycol, 180mM Hexanediol, VAPOR DIFFUSION, SITTING DROP, temperature 300.15K |