4HW9
Crystal Structure of Helicobacter pylori MscS (Closed State)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-03-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 113.920, 143.100, 178.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.563 - 4.140 |
| R-factor | 0.2469 |
| Rwork | 0.244 |
| R-free | 0.30440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.766 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.081 | 4.250 |
| High resolution limit [Å] | 4.140 | 4.140 |
| Rmerge | 0.755 | |
| Number of reflections | 20129 | |
| <I/σ(I)> | 2.2 | |
| Completeness [%] | 89.0 | 98.4 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 100 mM citrate pH 5.0, 200 mM lithium chloride, 28% PEG 400, 5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
