4HW0

Crystal structure of Sso10a-2, a DNA-binding protein from Sulfolobus solfataricus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-08
Detector	ADSC QUANTUM 210
Wavelength(s)	0.933
Spacegroup name	P 21 2 2
Unit cell lengths	60.221, 69.538, 80.727
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.360 - 2.000
R-factor	0.2065
Rwork	0.205
R-free	0.24490
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.714
Data scaling software	SCALA (CCP4_3.3.20)
Phasing software	CRANK
Refinement software	REFMAC (refmac_5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.360	40.360	2.110
High resolution limit [Å]	2.000	6.320	2.000
Rmerge	0.039	0.020	0.480
Total number of observations		10150	30661
Number of reflections	21998
<I/σ(I)>	39.6302
Completeness [%]	93.3	99.05	95.21
Redundancy	13.46	12.2	9.57

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	Succinic acid, HEPES, PEG MME 2000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K