4HVM
Crystal structure of tallysomycin biosynthesis protein TlmII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 123.799, 128.015, 129.307 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.711 - 2.704 |
| R-factor | 0.1843 |
| Rwork | 0.182 |
| R-free | 0.23220 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.628 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.720 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.090 | 0.957 |
| Number of reflections | 56697 | |
| <I/σ(I)> | 21.4 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.1 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 297 | 0.32M Ammonium Sulfate,0.1M HEPES, 22% PEG3350, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
