4HVL
Structure of a serine protease MycP1, an essential component of the type VII (ESX-1) secretion system
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-06-11 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.950, 73.080, 75.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.920 - 2.000 |
R-factor | 0.1709 |
Rwork | 0.169 |
R-free | 0.20640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1thm |
RMSD bond length | 0.010 |
RMSD bond angle | 1.310 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | BALBES |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.920 | 2.050 | |
High resolution limit [Å] | 2.000 | 8.950 | 2.000 |
Rmerge | 0.104 | 0.022 | 0.633 |
Number of reflections | 44283 | 496 | 3006 |
<I/σ(I)> | 11.41 | 43.9 | 1.39 |
Completeness [%] | 99.2 | 99.2 | 91.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 0.2M ZINC ACETATE, 0.1M IMIDAZOLE, 25% 1,2-PROPANEDIOL, 10% GLYCEROL, pH 8.0, vapor diffusion, hanging drop, temperature 295K |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 0.2M ZINC ACETATE, 0.1M IMIDAZOLE, 25% 1,2-PROPANEDIOL, 10% GLYCEROL, pH 8.0, vapor diffusion, hanging drop, temperature 295K |