4HVF
Crystal structure of green fluorescent protein lanGFP(Branchiostoma Lanceolatum)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.187, 105.802, 121.130 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.560 - 1.700 |
R-factor | 0.177 |
Rwork | 0.177 |
R-free | 0.21340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g3o |
RMSD bond length | 0.030 |
RMSD bond angle | 2.127 |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.770 |
High resolution limit [Å] | 1.700 | 3.680 | 1.700 |
Rmerge | 0.081 | 0.053 | 0.415 |
Number of reflections | 101261 | ||
<I/σ(I)> | 10.2 | ||
Completeness [%] | 99.5 | 99.7 | 95.3 |
Redundancy | 7.3 | 7.2 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 20 mM Tris pH 8.0, 200 mM NaCl, 5 mM EDTA mixed with an equal amount of reservoir solutions: 0.2M MgCl2x6H2O, 0.1M BIS-Tris pH 5.5, 25% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |