4HV1
Laser-induced microfragmentation of lysozyme crystals allows X-ray nanodiffraction characterization of individual domains (lb4)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID13 |
Synchrotron site | ESRF |
Beamline | ID13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-12-01 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.835, 78.835, 37.095 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.740 - 2.300 |
R-factor | 0.1969 |
Rwork | 0.193 |
R-free | 0.27780 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.532 |
Data scaling software | SCALA (3.3.16) |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 55.745 | 39.417 | 1.600 |
High resolution limit [Å] | 1.519 | 4.800 | 1.519 |
Rmerge | 0.020 | 0.020 | |
Total number of observations | 8401 | 14978 | |
Number of reflections | 5552 | ||
<I/σ(I)> | 5.2 | 6.1 | 0.3 |
Completeness [%] | 97.8 | 99.9 | 84.9 |
Redundancy | 13.1 | 12.2 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | LB-nanofilms were generated by the Langmuir Blodgett (LB) technique and its variation, a modified Langmuir Schaeffer technique (LS). 40 mg/ml, 50 mM, sodium acetate pH 4.5, 0.9 NaCl 1:1 in 50 mM sodium acetate , VAPOR DIFFUSION, HANGING DROP, temperature 298K |