4HV0
Structure and Function of AvtR, a Novel Transcriptional Regulator from a Hyperthermophilic Archaeal Lipothrixvirus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.971 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.153, 59.550, 84.039 |
Unit cell angles | 90.00, 102.76, 90.00 |
Refinement procedure
Resolution | 81.920 - 2.600 |
R-factor | 0.21383 |
Rwork | 0.211 |
R-free | 0.25832 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.764 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.920 | 2.668 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.950 | |
Number of reflections | 14095 | |
<I/σ(I)> | 20.9 | 3 |
Completeness [%] | 98.8 | 90.2 |
Redundancy | 14 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 22-27% PEG4000, 0.1 M HEPES, pH 7.5, 5-10% isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 296K |