4HV0
Structure and Function of AvtR, a Novel Transcriptional Regulator from a Hyperthermophilic Archaeal Lipothrixvirus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.971 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.153, 59.550, 84.039 |
| Unit cell angles | 90.00, 102.76, 90.00 |
Refinement procedure
| Resolution | 81.920 - 2.600 |
| R-factor | 0.21383 |
| Rwork | 0.211 |
| R-free | 0.25832 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.764 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.920 | 2.668 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.950 | |
| Number of reflections | 14095 | |
| <I/σ(I)> | 20.9 | 3 |
| Completeness [%] | 98.8 | 90.2 |
| Redundancy | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 22-27% PEG4000, 0.1 M HEPES, pH 7.5, 5-10% isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
