4HUK
MATE transporter NorM-NG in complex with TPP and monobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-01 |
| Detector | ADSC QUANTUM 1 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 118.335, 118.335, 226.473 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.590 |
| R-factor | 0.30899 |
| Rwork | 0.307 |
| R-free | 0.34944 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.152 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 60.000 |
| High resolution limit [Å] | 3.590 |
| Number of reflections | 21726 |
| Completeness [%] | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 300 | PEG, pH 8, VAPOR DIFFUSION, temperature 300K |
