4HUK
MATE transporter NorM-NG in complex with TPP and monobody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-01 |
Detector | ADSC QUANTUM 1 |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 118.335, 118.335, 226.473 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 3.590 |
R-factor | 0.30899 |
Rwork | 0.307 |
R-free | 0.34944 |
Structure solution method | AB INITIO PHASING |
RMSD bond length | 0.013 |
RMSD bond angle | 2.152 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 60.000 |
High resolution limit [Å] | 3.590 |
Number of reflections | 21726 |
Completeness [%] | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 300 | PEG, pH 8, VAPOR DIFFUSION, temperature 300K |