4HUD
Structure of the bacteriophage T4 tail terminator protein, gp15.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-08-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 |
Unit cell lengths | 100.682, 100.682, 155.891 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.956 - 2.700 |
R-factor | 0.2092 |
Rwork | 0.207 |
R-free | 0.25210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4huh |
RMSD bond length | 0.010 |
RMSD bond angle | 1.298 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.078 | 0.270 |
Number of reflections | 45176 | |
<I/σ(I)> | 20 | 3.3 |
Completeness [%] | 93.0 | 85 |
Redundancy | 5.5 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 301 | 0.15 M ammonium sulfate, 10.5 % isopropanol, 0.085 M Na Hepes pH 7.5, 20% PEG 4000, 15% glycerol., VAPOR DIFFUSION, HANGING DROP, temperature 301K |