4HU8
Crystal Structure of a Bacterial Ig-like Domain Containing GH10 Xylanase from Termite Gut
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.075 |
Spacegroup name | P 1 |
Unit cell lengths | 55.685, 86.963, 246.433 |
Unit cell angles | 92.96, 90.86, 108.56 |
Refinement procedure
Resolution | 49.080 - 2.000 |
R-factor | 0.22115 |
Rwork | 0.219 |
R-free | 0.26624 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nq6 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.098 | 0.603 |
Number of reflections | 293766 | |
<I/σ(I)> | 15 | 2 |
Completeness [%] | 97.7 | 96 |
Redundancy | 4.1 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 280 | 0.2 M potassium sodium tartrate tetrahydrate, 0.1 M sodium citrate tribasic dihydrate, 2 M ammonium sulfate, 20% glycerol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 280K |