4HU0
Crystal Structure of a metagenome-derived cellulase Cel5A in complex with cellotetraose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 6C1 |
Synchrotron site | PAL/PLS |
Beamline | 6C1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-01 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 21 3 |
Unit cell lengths | 136.924, 136.924, 136.924 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.300 - 2.380 |
R-factor | 0.154 |
Rwork | 0.152 |
R-free | 0.18800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.032 |
RMSD bond angle | 2.528 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.470 |
High resolution limit [Å] | 2.380 | 2.380 |
Number of reflections | 34430 | |
Completeness [%] | 100.0 | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 291 | PEG 4000, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |