4HTY
Crystal Structure of a metagenome-derived cellulase Cel5A
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-29 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97916, 0.97933, 0.96394 |
Spacegroup name | P 21 3 |
Unit cell lengths | 137.771, 137.771, 137.771 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.120 - 2.000 |
R-factor | 0.155 |
Rwork | 0.154 |
R-free | 0.16900 |
Structure solution method | MAD |
RMSD bond length | 0.041 |
RMSD bond angle | 2.798 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 59067 | |
Completeness [%] | 99.9 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 291 | PEG4000, MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |